Paris - Everton, the lone British representative remaining in the Europa League, were given a testing last-16 tie against Ukrainian outfit Dynamo Kiev after the draw was made on Friday in Switzerland.
Roberto Martinez's side have not reached the later stages of a European competition since they won the 1985 Cup Winners' Cup, but will have to earn a result in war-torn Ukraine to reach the quarter-finals.
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Fiorentina host Roma, in the first leg of their all-Italian tie, while holders Sevilla will be on the road at Villareal on March 12 for the first match in a battle of La Liga hopefuls.
Fiorentina coach Vincenzo Montella is a former player and coach with Roma while Villareal coach Marcelino was fired by Sevilla in his first season in 2012.
"It will be an open tie but I think that both teams would have chosen not to have faced each other, but Sevilla are just as difficult as any other team we could have been drawn against," Marcelino told Uefa.com.
"We will be playing against the champions, but we know that both teams are very evenly matched. It will be very difficult, but we are not very far away from their level of quality.
"I said yesterday that I would prefer any other team than a Spanish side as it's more attractive for the players if they play against a team from somewhere else in Europe." he added.
Another attractive tie features three-time winners Inter Milan at Wolfsburg in the first matchup between the Serie A giants and second-placed Bundesliga side.
"It will be a tough match and the round of 16 matches are always tricky and attractive," said Inter coach Roberto Mancini.
"We will work to be at our best. They are the second best team in the Bundesliga, and they also recently won 4-1 against Bayern." explained the former Manchester City manager.
Napoli, who were inspired to UEFA Cup victory by Diego Maradona in 1989, host Dinamo Moscow while the fifth Italian team in the draw, Torino, travel to Russia and a first-leg matchup against 2008 winners Zenit St. Petersburg.
Ajax, the only domestic champions in the last-16 and 1992 winners, also travel to the Ukraine and a difficult test against Dnipro Dnipropetrovsk.
Ukrainian and Russian teams were kept apart following a UEFA decision over the unrest between the two neighbors.
Finally, Belgian outfit Club Brugge host Besiktas, after the Istanbul club ousted Liverpool 5-4 on penalties, the match having finished 1-1 on aggregate.
"We might regard this as a good draw but any team who reach the round of 16 are good," said Besiktas president Fikret Orman.
"If we play like we did like yesterday, any team would be ok for us. We would like to go all the way in the UEFA Europa League.
The return leg in Turkey coincides with the 112th anniversary of the club's foundation.
Everton (ENG) v Dynamo Kiev (UKR)
Dnipro Dnipropetrovsk (UKR) v Ajax (NED)
Zenit St. Petersburg (RUS) v Torino (ITA)
VfL Wolfsburg (GER) v Inter Milan (ITA)
Villarreal (ESP) v Sevilla (ESP)
Napoli (ITA) v Dinamo Moscow (RUS)
Club Brugge (BEL) v Besiktas (TUR)
Fiorentina (ITA) v Roma (ITA)
The first legs will be played on March 12 with the return fixtures on March 19 and the final set to be staged at Warsaw on May 27.